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Dr Jonathan Golding

Senior Lecturer In Health Sciences

School of Life, Health & Chemical Sciences

Biography

Professional biography

I did my PhD research at King's College, London.

Post-doctoral positions at King's College London, Guy's Hospital, MRC National Institute for Medical Research, MRC Clinical Sciences Centre (Imperial).

MRC Collaborative Career Development Fellowship in Stem Cell Research.

Research interests

My lab works mainly on improving the effectiveness of the anti-cancer treatments photodynamic therapy (PDT) and radiotherapy. A full list of my research publications can be found at ORCID or Researchgate

I am the convenor of the OU Cancer Research Group.

A two-year clinical trial of our new combination PDT, in collaboration with the University of Cambridge, began in October 2013. On the basis of the excellent trial results, this has been adopted as a standard treatment option by Cambridge Veterinary School. 

2020: £16k OU funding to run a larger equine clinical trial, with a view to making the combination PDT technique a standard treatment option throughout the UK.

PI. Clinical trial of combination photodynamic therapy for equine sarcoids . European Society for Veterinary Dermatology (2013-2015). €20k

PI. Mechanisms of nanoparticle toxicity in normal and tumour cells. Industrial funding (2015-2018). £68k

Co-I. Targeting radiotherapy with DNA binding metal complexes and their attachment to gold nanoparticles. Royal Society of Chemistry (2015). £5k

Co-I. EU Framework 7 grant PEOPLE-2013-ITN ARGENT- Advanced Radiotherapy, Generated by Exploiting Nanoprocesses and Technologies (2014-2017). £3.65M

Patent: Nanoparticles and their uses in medicine. GB1423049.4 (23/12/2014)

YouTube

 

Teaching interests

My main teaching interest is in developing global online life sciences education.

SK297 production and presentation module chair (Infection, immunity and public health)

SK320 production and presentation module chair (Infectious disease and public health)

S390 module chair (STEM capstone project module)

Module author for T366 (Engineering on the Nanoscale)

External collaborations

I collaborate with:

  • Clinicians at Milton Keynes University Hospital and Northampton Hospital.
  • Radiographers at GenesisCare, Milton Keynes.
  • Veterinary surgeons at the University of Cambridge and Nottingham Trent University
  • Pharmaceutical companies (Midatech Pharma, Glaxo Smith Kline).
  • New University of Lisbon (RaBBiT doctoral training program)

International links

As a member of the Affiliated Research Centre Management Group, I work to ensure the excellent and equivalent provision of PhD student training across the world in our network of OU Affiliated Research Centres (ARCs) and to foster research relationships between to OU and ARCs.

Projects

Development of H19-targeting antisense oligonucleotides for DIPG therapy

Diffuse intrinsic pontine glioma (DIPG) is the most frequent paediatric brainstem malignancy, and it is invariably associated with a dire prognosis [1]. DIPG is characterized by diffuse infiltrative growth patterns and by an invasive phenotype that underpins its inadequate response to both pharmacological and surgical treatments. DIPG response to radio- or chemo- and targeted therapies is short lived at best. Hence, it is of paramount importance to identify new targets for the development of effective therapies. The genomic landscape of DIPG has been recently described as an heterogenous collection of mutations and copy number variations in protein-coding genes. The most frequent mutation occurs at histone H3 lysine 27 (H3K27M) resulting in widespread epigenetic deregulation. Despite intensive research, no effective protein-targeting therapy has been developed for DIPG. Long noncoding RNAs (lncRNAs) are non-protein coding transcripts longer than 200bp. For several decades, lncRNAs have been considered non-functional transcriptional noise. Recent deep sequencing data have revealed that lncRNAs represent a vast and largely uncharted region of the human transcriptome (more than 50,000 unique loci, 100nMol), broad CNS distribution and rapid cellular uptake. Antisense oligonucleotides (ASOs) are clinically approved molecules that target and silence specific RNA sequences. Our group has shown that antisense ASOs can efficiently target oncogenic lncRNAs. In this project, we will to develop lncRNA-targeting ASOs to stop DIPG proliferation, with the final goal of developing personalised therapies for this incurable disease

Mechanisms of nanoparticle toxicity

This is a matched funded PhD student project between LHCS and Midatech. This project will discover why certain nanoparticles are selectively toxic to different cell types.

Improving the efficiency and targeting of radiosensitisers

New University of Lisbon have a doctoral training program called RaBBiT, whereby the student does research towards their PhD in a host lab (in this case at the OU). The student will be a visitor to the OU for 2 years. The Sir John Mason Academic Trust will meet the cost of the project specific consumables and expenses.

Publications

Book Chapter

New Research in Ionizing Radiation and Nanoparticles: The ARGENT Project (2016)

Journal Article

REST-dependent glioma progression occurs independently of the repression of the long non-coding RNA HAR1A (2024)

Marine Natural Products with Activities against Prostate Cancer: Recent Discoveries (2023)

The Long Non-Coding RNA H19 Drives the Proliferation of Diffuse Intrinsic Pontine Glioma with H3K27 Mutation (2021)

Galactose:PEGamine coated gold nanoparticles adhere to filopodia and cause extrinsic apoptosis (2019)

A comparison of the radiosensitisation ability of 22 different element metal oxide nanoparticles using clinical megavoltage X-rays (2019)

Photodynamic therapy and diagnosis: Principles and comparative aspects (2018)

Glycolysis inhibition improves photodynamic therapy response rates for equine sarcoids (2017)

Cancer-selective, single agent chemoradiosensitising gold nanoparticles (2017)

Gold nanoparticles for cancer radiotherapy: a review (2016)

Engineered neural tissue with aligned, differentiated adipose-derived stem cells promotes peripheral nerve regeneration across a critical sized defect in rat sciatic nerve. (2015)

A 3D in vitro model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury. (2013)

Engineered neural tissue for peripheral nerve repair (2013)

Targeting tumour energy metabolism potentiates the cytotoxicity of 5-aminolevulinic acid photodynamic therapy (2013)

Fully protected glycosylated zinc (II) phthalocyanine shows high uptake and photodynamic cytotoxicity in MCF-7 cancer cells (2013)

Engineering an integrated cellular interface in three-dimensional hydrogel cultures permits monitoring of reciprocal astrocyte and neuronal responses (2012)

Regulation of PP2A activity by Mid1 controls cranial neural crest speed and gangliogenesis (2012)

Alignment of astrocytes increases neuronal growth in three-dimensional collagen gels and is maintained following plastic compression to form a spinal cord repair conduit (2010)

Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: a longitudinal imaging study (2010)

A versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis (2009)

Developing a 3D culture model of CNS myelination (2009)

Astrocyte alignment increases neurite outgrowth in a 3D cell culture model (2009)

Skeletal muscle stem cells express anti-apoptotic ErbB receptors during activation from quiescence (2007)

Increased GFAP immunoreactivity by astrocytes in response to contact with dorsal root ganglia cells in a 3D culture model (2007)

Mouse myotomes pairs exhibit left-right asymmetric expression of MLC3F and α-skeletal actin (2004)

Heparin-binding EGF-like growth factor shows transient left-right asymmetrical expression in mouse myotome pairs (2004)

Myf5 expression in satellite cells and spindles in adult muscle is controlled by separate genetic elements (2004)

Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? (2004)

Roles of erbB4, rhombomere-specific, and rhombomere-independent cues in maintaining neural crest-free zones in the embryonic head (2004)

ErbB4 signaling during breast and neural development: novel genetic models reveal unique ErbB4 activities (2003)

Neural and mammary gland defects in ErbB4 knockout mice genetically rescued from embryonic lethality (2003)

Cues from neuroepithelium and surface ectoderm maintain neural crest-free regions within cranial mesenchyme of the developing chick (2002)

Presentation / Conference

Practical or data-based projects? Types of undergraduate capstone projects chosen by distance-learning biology and environmental science students at the Open University (2022)

Cancer-selective toxicity of gold nanoparticles: effects of synthesis time and charge (2016)

Selective cancer cell toxicity and radiosensitization using different high atomic number nanoparticles (2016)

Selective cancer cell toxicity and radiosensitization with gold nanoparticles (2015)

Fabrication of an endoneurium using engineered neural tissue within a peripheral nerve repair conduit (2013)

A nerve repair conduit containing differentiated adipose-derived stem cells within engineered neural tissue can support and guide neuronal growth in vitro and in vivo (2012)

A living replacement tissue for peripheral nerve that can enhance regeneration in vitro and in vivo (2012)

Engineered neural tissue with aligned Schwann cells supports neuronal regeneration in vivo and can be assembled using differentiated adipose-derived stem cells (2012)

Astrocytes expressing GFP in 3D collagen gels provide an effective model for screening the glial response to potential CNS cell therapies (2012)

Engineered neural tissue with columns of aligned Schwann cell-like cells from differentiated adipose-derived stem cells can support and guide neuronal growth in vitro (2012)

Aligned Schwann cells within 3D tissue-like gels provide guidance to regenerating neurites (2011)

Aligned cellular and acellular collagen guidance substrates for peripheral nerve repair (2011)

A tissue engineered collagen conduit containing columns of aligned Schwann cells supports neuronal regeneration in vitro (2011)

Development of an integrated collagen gel system for studying cellular interfaces following spinal cord injury (2010)

Schwann cells in collagen gels survive plastic compression and maintain their alignment: development of a cellular biomaterial for peripheral nerve repair (2010)

Plastic compression of aligned cellular collagen gels for nervous system repair (2010)

Potentiation of AlPcS2 mediated photodynamic therapy by energy metabolism inhibitors in human tumour cell lines (2009)

Astrocyte alignment in 3D collagen gels increases neurite outgrowth; implications for improving spinal cord repair (2009)

Monitoring reactive gliosis in 3-dimensional astrocyte cultures; a model of spinal cord damage (2008)

Glucosamine improves the efficiency of photodynamic therapy (PDT) (2008)

Midline1 and the development of the cranial peripheral nervous system (2008)

Modelling the injured spinal cord using 3-dimensional cell cultures; strategies for improving tissue engineered repair (2008)

Modelling of the injured spinal cord using 3-dimensional cell cultures; strategies for improving tissue engineered repair (2007)

An in vivo model of epidermal neural crest cell migration after implantation into the brain (2007)

Development of a 3-dimensional in vitro model to study reactive gliosis following nervous system injury (2007)

Increased GFAP immunoreactivity by astrocytes in response to contact with dorsal root ganglia cells in a 3D culture model (2007)

Maintaining the regenerative compartment of adult skeletal muscle (2005)

Not all activated satellite cell progeny commit to differentiation (2003)